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2
CONTENTS
Components of the Kit .......................................................................................3
Quality Control ....................................................................................................4
Safety Warnings and Precautions ..............................................................4, 26
Introduction .........................................................................................................5
Materials Not Supplied.......................................................................................8
Protocol ...............................................................................................................9
Supplementary Information.............................................................................11
General guidelines .........................................................................................11
Preparation of template DNA.........................................................................12
Cycle conditions and template quantity.........................................................12
Cycling temperatures ..................................................................................... 12
Number of cycles and quantity of template ...................................................13
Designing a new sequencing primer .............................................................13
Sequencing PCR products ............................................................................15
Elimination of compressions ..........................................................................15
Reading farther from or closer to the primer .................................................17
Denaturing gel electrophoresis......................................................................18
Troubleshooting ...............................................................................................20
Control DNA Sequence ....................................................................................22
References ........................................................................................................23
Related Products ..............................................................................................24
Contact Information .........................................................................................25
- Thermo Sequenase 1
- Radiolabeled Terminator 1
- Cycle Sequencing Kit 1
- CONTENTS 2
- COMPONENTS OF THE KIT 3
- QUALITY CONTROL 4
- INTRODUCTION 5
- Chain termination sequencing 6
- MATERIALS NOT SUPPLIED 8
- PROTOCOL 9
- SUPPLEMENTARY INFORMATION 11
- Preparation of template DNA 12
- Cycling temperatures 12
- 0.5pmol 1pmol 2pmol 8pmol 14
- 300 bases 14
- 150 bases 14
- Sequencing PCR Products 15
- Elimination of compressions 15
- Gel electrophoresis reagents 18
- TROUBLESHOOTING 20
- CONTROL DNA SEQUENCE 22
- REFERENCES 23
- RELATED PRODUCTS 24
- USB CORPORATION 25
- Material safety data sheet 26
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